Pci neo vector promega

Article Snippet: The cDNA coding for FGFR2c was amplified by pMD18T-simple FGFR2c (HG10824-M, Sino Biological Inc., Beijing, China) and subcloned in pCI-neo expression vector using standard procedures (Promega, Madison, WI) and the resulting pCI-neo FGFR2c construct was verified by sequencing.

Contains the CMV immediate-early enhancer/promoter region and is designed to promote Name: pCI-neo. Description: Mammalian expression vector with CMV promoter, T7 and T3, chimeric intron; amp resistance (neomycin for mammalian selection); Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction, Name: pCI-neo, Type: plasmid, Supplier: Promega. Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) 18 Feb 2016 The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus ( CMV) immediate-early enhancer/promoter region to promote pCI-neo Mammalian Expression Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or 2009 J. Biol. Chem. See all citations. Similar Products.

20.10.2020

Show more If you are using MCLAB's DNA sequencering services, you do not need to purchase or submit any primers listed here in your orders. You only need to specify the primer names in your order form and MCLAB will take care of everything related to these primers. Belgium). Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes.

pCI-neo Mammalian Expression Vector 20µg E1841 The pCI-neo Vector is supplied frozen at a concentration of 1mg/ml in TE buffer (pH 8.0). Storage Conditions:Store the pCI-neo Mammalian Expression Vector at –20°C. 3. Vector Components 3.A. Enhancer/Promoter Regions The CMV immediate-early enhancer/promoter region present in the pCI-neo

DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation.

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.

This vector also contains the neomycin phosphotransferase gene, a … This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.

Multiple cloning sites exist for easy insertion of cDNA. References This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.

pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 Plasmid pCI-neo:alpha4-eGFP from Dr. Henry Lester's lab contains the insert alpha4-eGFP and is published in J Gen Physiol. 2011 Jan;137(1):59-79. This plasmid is available through Addgene.

pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 pCI 4,006 Y T7 N Y Y T7 EEV pCI-neo 5,472 Y T7, T3 N Y Y T3, T7 EEV Key: S - SP6 M - M13 *Note: The T7 EEV (Eukaryotic Expression Vector) Promoter Primer (Cat.# Q6700) is designed specifically for use with Promega’s mammalian expression vectors. pCI-neo-ER vector was constructed by insertion of human ER cDNA derived from pIC-ER-F (ATCC, Manassas, Virginia, U.S.A.) in pCI-neo mammalian expression and neomycin resistant vector (Promega, Madison, Wisconsin, U.S.A.). pGL3-estrogen respon-sive element (ERE) luciferase reporter vector, pGL3-ERE, was constructed by insertion of four copies of Lane 20.

PN101_17252_Final.qxp 2/6/2009 9:07 AM Page 8. PROTEIN EXPRESSION PROMEGA NOTES WWW.PROMEGA.COM NUMBER 101 FEBRUARY 2009 9 CONCLUSION Mar 08, 1999 Name: pCI-neo: Description: Mammalian expression vector with CMV promoter, T7 and T3, chimeric intron; amp resistance (neomycin for mammalian selection); restriction enzyme cloning. Promega provides the FuGENE® HD Protocol Database to help identify a protocol for your cell line when using the FuGENE® HD Transfection Reagent. A drop-down menu allows you to search the database by cell line, plate type and number of cells to be transfected. pCI-neo Mammalian Expression Vector (Cat.# E1841)] can be selected for stable vector and the DsRed-Golgi expression vector.

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The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

KpnI sites of the pCI-neo vector (Promega). The expression of prME was under the control of the cytomegalovirus (CMV) promoter. For the pCI-D2prME-VSVG, pCI-D2prME-CD4 pCI-ZprMEVSVG, or pCI-ZprMECD4 vector, the transmembrane and cytoplasmic domain (TM) of the DENV2 or ZIKV E gene were replaced with the TM gene of the VSV G or the CD4 pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression.